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Chao, A

Prostaglandin E2 stimulates adaptive IL-22 production and promotes allergic contact dermatitis

Evidence from germ-free mouse models demonstrates an essential role for the microbiota in ENS maturation and function, with the absence of commensal colonization leading to reduced enteric neuronal density, impaired intestinal peristaltic reflexes (106108), and defective recruitment of EGCs into the intestinal mucosa during development, ultimately compromising neuroimmune integration and homeostatic regulation (109)

Every 5th fraction was combined into a new Eppendorf to make five final fractions that were dried using a SpeedVac vacuum concentrator