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London, UK: BMJ Group, RPS Publishing, 2009 17

Indeed, GSH, Trx, Grx, Prx (together with the enzymes catalyzing their reduction), and the enzymatic antioxidant defense, as a whole, concur to modulate either ROS concentration, or the availability of cellular thiols, thus regulating the function of a large number of proteins implicated in the induction and/or execution of redox-sensitive pathways

Membranes were incubated with the appropriate primary antibody overnight at 4 C

Knowing " what does reduced glutathione do?" and understanding the significance of the GSH : GSSG ratio gives you insight into how your body responds to stress and disease