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ELISA The levels of tumor necrosis factor (TNF)-, interleukin (IL)-1, IL-6 and monocyte chemoattractant protein (MCP)-1 in serum and liver tissue samples were determined by ELISA kits (R&D systems) [51], The levels of TGF- and IL10 in serum were determined by ELISA kits (R&D systems) [52]

Of 492 patients identified, 382 (78%) were receiving injections more frequently than the recommended three monthly dose of hydroxocobalamin

In contrast, no specific patterns were seen in latency measurements although latency did decrease over time in a consistent and similar manner across all groups (Fig