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Cell Stem Cell 10 , 556569 (2012)

After sorting with anti-CD45 magnetic beads, the tumor cells were stained with anti-CD3, anti-CD8, anti-CD4, anti-CD25, anti-CD127, anti-PD-1, anti-TIM-3, and anti-Granzyme B antibodies for 30 min, fixed and permeabilized using a Foxp3/Transcription Factor Staining Buffer Set (eBioscience), then stained with anti-IFN- antibody for 30 min