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Faucheux, B

Yang R, Qu C, Zhou Y, Konkel JE, Shi S, Liu Y, et al

AOP Development Strategy Context This AOP was developed for the project "CHRONIC TOXICITY OF PESTICIDES IN DRINKING WATER IN PARABA (TRIGGER): IDENTIFYING THE TRIGGERS OF A SILENT EPIDEMIC," financed by the "Fundao de Apoio Pesquisa do Estado da Paraba (FAPESQ-PB)." The project aims to understand how oxidative stress and reproductive toxicity can be triggered in animals by aquatic pollutants, such as atrazine Strategy The first step in developing this AOP was to conduct a literature review to gather toxicological data on the herbicide atrazine's impact on aquatic vertebrates in relation to oxidative stress and reproductive toxicity

Glutathione activity assay (ELISA) The concentration of total glutathione, oxidized glutathione, ratio of reduced glutathione to oxidized glutathione were measured in OB extracts using the glutathione detection kit from Enzo Life Sciences (Enzo Life Sciences, East Farmingdale, NY, USA, ADI-900-160) following the manufacturer's instructions