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Nature 385 , 640644 (1997)

coli Generates substantial protein yields suitable for biochemical and structural studies GST Tag for Easy Purification GST tag binds specifically to glutathione resin , allowing simple one-step purification Reduces contamination and eliminates multi-step chromatography Flexible Cloning Multiple Cloning Sites (MCS) provide versatility to insert various genes of interest Compatible with standard molecular biology workflows Versatile Applications Protein-protein interaction studies (e.g., pull-down assays) Enzyme activity assays and functional studies Structural biology : crystallography, NMR, and other biophysical analyses How pGEX Vectors Work Gene Cloning: Insert your gene of interest into the vectors MCS Transformation: Introduce the plasmid into E

Monath et al, 2002

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