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Cells were next cultured for an additional 2 weeks-period in the same medium supplemented with 2% (vol/vol) dimethyl sulphoxide (DMSO), in order to get a full differentiation status of the cells ( 40 ) before their use for metabolism activity assay

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Side effects and precautions Vitamin B12 injections are generally safe, but some side effects have been reported

These water molecules are absent from the structure of the Meisenheimer complex bound to GST, implicating that deprotonation of the cysteine occurs during formation of the ternary complex which involves expulsion of the inner bound water molecule