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NO is an active molecule produced by the nitric oxide synthase (NOS) protein family, which directly binds and inactivates iron-containing enzymes [48] or reacts with superoxide anion radical O2 to form a highly active peroxy sulfoxide Nitrates (ooo-), which can attack membrane lipids and proteins of the pathogens, especially protein thiols [49]

Opening of mitochondrial K(ATP) channels triggers the preconditioned state by generating free radicals

it has a free thiol group, which is likely to confer particular properties on functional sites of proteins that contain this highly conserved residue

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