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Store lyophilized material at 4 F (20 C) or below Allow vial to reach room temperature before opening Protect from light, heat, and humidity Use sterile laboratory equipment during preparation Avoid repeated freezethaw cycles Label reconstituted samples with preparation date and concentration Following these practices supports reliable peptide handling and experimental reproducibility

Figure 6 3.4 Expression of pro- and anti-inflammatory cytokines Western blot analysis was used to assess the levels of pro-inflammatory (TNF- and IL-12) and anti-inflammatory (IL-4 and IL-10) cytokines at the site of injury and the lumbar enlargement in the SCI and DCM models

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Dispose of used syringes in the sharps container and return it to the clinic