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Cultures were passaged by trypsinization (0.05% trypsin-0.53 mM EDTA) every week and were seeded at a density of 1.2 10 4 cells/cm 2 in 60 20 mm or 100 20 mm diameter culture dishes (Sarsted, Nmbrecht, Germany), 96-well or 24-well plates (Sarstedt), or 15 -slide 4-well coverslip (Ibidi USA, ThermoFisher Scientific) for subsequent experiments

Similarly, increased oxidative stress was not detected in several Drosophila knockdown models, and overexpression of ROS scavengers did not rescue the viability, heart dysfunction or neurodegeneration phenotypes (Anderson et al., 2005

Furthermore, GPX2 functions as a temporal gatekeeper of cell fate decisions during endoderm differentiation, with distinct stage-specific roles

Anxiolytic-like effect of a salmon phospholipopeptidic complex composed of polyunsaturated fatty acids and bioactive peptides