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Vitamin B12 deficiency symptoms If you are deficient in vitamin B12, you may experience a range of symptoms such as: Megaloblastic anemia, a condition where the red blood cells are larger than normal and cant function properly Fatigue and weakness Low energy levels Heart palpitations Loss of appetite and weight loss Constipation Soreness of the mouth or tongue Numbness and tingling in the hands and feet Difficulty maintaining balance Depression Confusion Poor memory Dementia

the shRNA was transfected into cells using the lipofectamine method described above