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Annexin-V binding can happen in two cases: when phosphatidylserine is externalized to the outer layer of the cell membrane or when pores in the cell membrane are formed

Furthermore, insulin stimulated an increase in glutathione levels in wild-type MEFs in a manner completely sensitive to rapamycin, an effect ablated in Atf4 knockout cells, which had a very low abundance of glutathione (Figure 7G)

Additionally, its potential to reduce reactive oxygen species (ROS) in dermal layers makes it an attractive candidate for studying oxidative stress modulation

Taken together, these data indicate that nitrate-derived NO prevents scavenging of its major storage form by inhibitory S -nitrosylation of GSNOR1