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In order to further confirm the bioactivity of these recombinant protein, dot blot was also carried out wherein increasing concentration of FAP174 on the blot was overlayed with CrFAP65AH1, Cr FAP65AH2 and their respective variants

1 Introduction Neurodegenerative diseases are irreversible disorders of the nervous system caused by progressive loss of neurons in the brain and spinal cord ( In recent decades, studies have identified several key factors contributing to neurodegeneration, including inflammation, aging, oxidative stress, and genetic mutations ( Over the past few decades, multiple modes of cell death have been defined