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Light dark activity box is a widely used test to detect the anxious state of rodents [68], and this result was in accordance with a previous study by [69]

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This hormonal preservation is especially relevant for individuals with existing endocrine considerations, those prone to stress-related eating, or anyone seeking to avoid the systemic hormonal disruptions that often accompany aggressive fat loss interventions

A study with a population of asymptomatic B 12 -deficient elderly Chileans showed that patients displayed improved function of myelinated peripheral nerves after vitamin B 12 treatment and a positive association with folate status (Brito et al., 2016)