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An instrument with a 400-W (or higher) capacity is recommended, for example from Branson ( After low-speed centrifugation using standard preparative centrifuges (Beckman Coulter Avanti Series can be found at high-speed centrifugation is a convenient and rapid cleanup step before column chromatography (Fig

3.3.2 Excitatory amino acid transporters Glutamate uptake is accomplished in the CNS by excitatory amino acid transporters (EAATs) that are present in the pre- and postsynaptic cell surface of neurons and astrocytes, where glutamate is preferentially recycled (163)