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However, persistent oxidative stress and endothelial injury may promote pathological remodeling despite this acute activation

Results Genome-wide CRISPR/Cas9 library screening combined with single-cell sequencing identified METTL14 as a determinant of CDK4/6i sensitivity To systematically identify the critical genes associated with CDK4/6i resistance in breast cancer, we transduced T47D cells (ER + breast cancer cells) with a genome-wide CRISPR single-guide RNA (sgRNA) library consisting of 70,290 sgRNAs targeting every coding isoform (Fig

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