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The effect of liquid application on global gene expression, stress-responsive signaling protein phosphorylation, growth factor secretion, pro-inflammatory cytokine secretion, trans-epithelial electrical resistance (TEER), and permeability to a 20 kDa fluorescent dextran was then determined immediately after 6 and 24 h of liquid application (Figure 1A)

The rate in the last two decades, however, is nearly double that of the last century

But the Royal College of Physicians has previously called for the devices to be made more widely available, arguing smokers should be moved on to safer substitutes such as e-cigarettes to supplement therapeutic approaches using nicotine patches and gum

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