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Middle Phase (Days 7-21) Pain reduction and visible healing progress typically become noticeable within the 5-10 day range

But at the beginning of the culture, when cells were plated (6 hours) and when they were confluent (day 5), most cells were in the G0/G1 phase of the cell cycle

Likewise, Miller and Sunde (137) stated that an abrupt increase in air temperature negatively affects eggshell quality within just one oviposition cycle, highlighting that respiratory alkalosis in hens sharply reduces blood ionized calcium levels (133)

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