AFM was invented in 1985 by IBM scientists Gerd Binnig, Calvin Forrest Quate (19232019), and Christoph Gerber, who reported a development of a new type of microscope, which, being a combination of the principles of the scanning tunneling microscope [STM that was invented in 1981 by Gerd Binnig, Heinrich Rohrer (19332013), Christoph Gerber, and Edi Weibel ( Biomedical applications of AFM, which can operate in ambient air, high vacuum, and liquid environments, are centered at topographic imaging [e.g., it can resolve DNA double helix (Mou et al., 1995) or probe structure and dynamics of nucleosomes (Stumme-Diers et al., 2019), or visualize and provide topological information about amyloid fibrils (Lutter et al., 2020
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From experimental data of kinetic measurements of BRET, following vehicle and baseline corrections, quantification of induced responses from individual peptide concentrations was determined by calculating the net area under curve from the 10 min response (AUC 0-10min) ) in GraphPad Prism version 9.0.1 (GraphPad Software Inc., San Diego, CA)
