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The solubilization of the tetrazolium salt was carried out by adding 1.4 mL of buffer prepared from 10% Triton X-100 and 0.04 M HCl in isopropanol and keeping the sealed plates for 24 h in the dark at 30C and 100 rpm

RNA Extraction Total RNA was extracted by using the Qiagen tissue extraction kit, Qiagen, Cat

Ferroptosis inhibitors successfully mitigate radiation injury across several animal models and organ systems, demonstrating again the universality of ferroptosis involvement in IR