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Injection of seminal fluid into the hemocoel of honey bee queens ( Apis mellifera ) can stimulate post-mating changes

This result was confirmed by Western blotting of GYS1 and GYS2 in HuH-7 cells compared with liver tissue extracts

After cell lysis and centrifugation, the cleared supernatant was applied to an equilibrated 5 mL PureCube Ni 2+ -NTA column and washed extensively with high salt washing buffer (20 mM Tris-HCl, 500 mM NaCl, 10 mM or 20 mM imidazole, 2 mM -mercaptoethanol)

Evolution of the human mitochondrial ABCB7 [2Fe-2S](GS)(4) cluster exporter and the molecular mechanism of an E433K disease-causing mutation