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Interestingly, the screening revealed the existence of an allosteric pocket close to the NADPH binding site, named the doorstop pocket since ligands binding at this site interfere with TR activity by hampering the opening movement needed to allow cofactor binding

and IEP, 0.67 (Fig

Levofloxacin persisters (levopersisters) were quantified as CFU/mL, and minimum inhibitory concentrations (MICs) were assessed using the broth microdilution method, following the Clinical and Laboratory Standards Institute (CLSI) guidelines [21]