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Determination of the molecular mass and oligomerization state of purified recombinant proteins The molecular masses of purified recombinant proteins were analyzed using a Bruker microTOF-Q spectrometer (Bruker Daltonics, Bremen, Germany) equipped with an Apollo II electrospray ionization source as described previously (Couturier et al., Enzymatic activities The GSH-conjugation activity toward phenetyl isothiocyanate (PITC), benzyl isothiocyanate (BITC), 1-chloro-2,4-dinitrobenzene (CDNB), 4-hydroxy-2-nonenal (HNE), 4-nitrophenyl butyrate (PNP-butyrate) was assayed at 25C by following absorbance at 274 nm for isothiocyanate derivatives, or at 224, 340, 412 nm for HNE, CDNB, and PNP-butyrate respectively

(58) Ballard, J

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It ultimately affects the balance of glutamate between synapses and neurons, leading to neuronal excitotoxic death