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In the no-gap trials, the background noise was a 70-dB SPL pure tone at 16 kHz, followed by a 50-ms white noise burst at 110 dB SPL to elicit the startle response

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Therefore, results require some time and vary for each skin concern, such as: Skin Glow: 3-4 weeks General body brightening or even skin tone: 6-8 weeks Stubborn hyperpigmentation like acne spots, melasma, dark patches: 2-3 months Overall skin tone change: visible improvements in 3-4 months Glutathione supplements for hyperpigmentation and dull skin tone need consistency and adoption of a healthy lifestyle to see noticeable positive results

After filtering the clarified cell lysate through a 0.45 m filter, it was loaded onto the column at a flow rate of 3 ml min 1