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429 These lipid metabolic changes provide biomarkers closely associated with disease onset and progression, offering critical support for early diagnosis and prognosis assessment

doi:10.1007/BF02537824

To generate nhr-80;fat-6 double mutants we crossed fat-6 males with nhr-80 hermaphrodites on 18:1 9 supplemented plates and isolated the F1 generation onto new supplemented plates