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The negative effects are mostly due to the injection process, which leads to a compromised shell and membrane of the egg, allowing for pathogenic bacteria and environmental factors affecting the egg and embryo

S.AminA

Furthermore, it is critically important to apply quantitative analyses, e.g., generalized mixed models for abundance trends, spatial autocorrelation metrics for connectivity, and time-series regressions for phenological shifts, to detect statistically significant changes and forecast vulnerability under climate change scenarios and human impacts

While feeding, aphids are also injecting their saliva, rich in proteins and other components known to impact the plant-aphid relationship