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HpGroEL variants were diluted into the ATPase reaction buffer (50 mM TrisHCl, 1 mM KCl/MgCl 2 , 250 M ATP, pH 7.5) to a final concentration of 1 M

Following the discovery of the binding capacity of IMiDs drugs with cereblon E3 ligase, the focus in this area over the last few years has been on the synthesis and study of new structural analogs of IMiDs with the aim of obtaining new and better building blocks for the construction of cereblon-recruiting PROTACs 43,53

Biochim Biophy Acta 582: 67-78

The platelet proteome analysis revealed 19 differential spots which were identified to be 19 platelet proteins, which included 10 cytoskeleton proteins, thrombin generation related proteins, and antioxidant enzymes