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The cells are contacted with a test substance and the receptor agonist (ligand), such as dATP, dAMP, or UTP, and the signal produced by the reporter gene is measured after a defined period of time

Furthermore, our data demonstrated that co-injections of D-CYSee (250 mol/kg, IV) and to a lesser extent, betaine (250 mol/kg, IV), markedly diminished the development of physical dependence induced by multi-day morphine injections (escalating daily doses of 1030 mg/kg, IV), as assessed by the lesser number of withdrawal phenomena elicited by the injection of the opioid receptor antagonist, naloxone (1.5 mg/kg, IV)