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Eight pH buffers (pH 6.0, 6.5, 7.0, 7.5, 8.0, 8.5 and 9.0) were used to detect the catalytic activity of rHrGSTm1, determined using phosphate buffers (100 mM) at pH 6.0, 6.5, 7.0, 7.5 and 8.0, and Tris hydrochloric acid buffers (100 mM) at pH 8.5 and 9.0

Methods: Control (n=3) livers were kept in 50 ml UW at 4 o C for 2 hr to simulate cold ischemia of procurement and back-benching, followed by 1 hr of simulation of graft implantation, achieved by gradually increasing temperature from 4 o C towards room temperature

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