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The binding of AGEs to their receptor (RAGE) activates the AGEs/RAGE signaling pathway, inducing inflammation, oxidative stress, cell apoptosis, insulin resistance, and damage to pancreatic cells 92

The use of carbon monoxide for diagnostic purposes, such as total haemoglobin mass measurements or the determination of pulmonary diffusion capacity, is not prohibited under the supervision of a medical or scientific professional

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Both the Tet-inducible vector pRMC2 (Addgene, plasmid 68940) 70 and the mKate insert were digested with KpnI-HF and EcoRI-HF (New England Biolabs), gel purified using a QIAquick PCR purification kit (Qiagen), and ligated using T4 DNA ligase (New England Biolabs) at 16 C for 16 h