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The M1 phenotype, stimulated by lipopolysaccharide (LPS) and interferon- (IFN-), was used as a positive control, whereas the M2 phenotype, induced by interleukin-4 (IL-4), functioned as a negative control

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The gap between doing it right and doing it wrong comes down to three things most guides never mention: temperature stability post-reconstitution, container selection for oxidation prevention, and the 28-day degradation curve that determines actual usable shelf life