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Am J Clin Pathol 116:263270 Buzzetti E, Petta S, Manuguerra R, Luong TV, Cabibi D, Corradini E, Craxi A, Pinzani M, Tsochatzis E, Pietrangelo A (2019) Evaluating the association of serum ferritin and hepatic iron with disease severity in non-alcoholic fatty liver disease

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Once the culture reached an optical density (OD) 600 of around 0.2, it was transferred to 18 C with shaking, and, once it reached an OD 600 of around 0.6, 0.5 mM of IPTG (GoldBio, I2481C50) was added