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As a consequence of immune defense and cell signaling mechanisms after infection, excessively generated ROS causes oxidative stress, a condition of imbalance between free radical generation and antioxidant defenses

For assessment of mitochondrial membrane potential in cultured cells, primary cultured tubular cells were seeded at the density of 5 10 4 cells/well on an 8-well open -slide chambered coverslip

Subsequent functional studies using Xenopus and mouse oocytes showed that this protein can transport various divalent cations, such as Cd 2+ , Zn 2+ , Mn 2+ , and Fe 2+ 27,57,58,59