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Microbiota-derived metabolites are key mediators of host-microbe interactions ( 2.3 Bidirectional neuroendocrine crosstalk In addition to regulating host metabolism and immune responses, the gut microbiota is recognized to play a critical role in the production of neurotransmitters and engages in complex bidirectional communication with the hypothalamicpituitarygonadal (HPG) axis, contributing significantly to the pathogenesis and potential treatment of PCOS

Therapeutically, we argue against indiscriminate antioxidant loading in favor of node-specific, compartment-targeted modulation (NOX/NOS tuning, mitochondrial QC/RET tempering, ER redox control, iron/ferroptosis management, calibrated sulfur-axis support), implemented as time-staged sequences and titrated to biomarkers

Materials and Methods Cell Culture U-87 MG cells were maintained in Eagles Minimum Essential Medium supplemented with 10% FBS and 1x pen-strep

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