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Assessment of antioxidant enzyme activities and oxidative stress markers To assess antioxidant enzyme activities and oxidative stress markers, frozen-thawed spermatozoa of each group (repeated in triplicated) were collected and centrifuged at 800g for 20 min to separate spermatozoa from seminal plasma

This approach simplifies experimental designs and enhances the efficiency of generating complex protein assemblies

doi: 10.1177/019262338701500219

The initial selection was done using the Students t -test and was based on a binary comparison (genes significantly different between LPS + BSO and LPS alone were selected)