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Intervention studies have shown that supplementation with riboflavin (the MTHFR cofactor) effectively lowers blood pressure, specifically in individuals with the variant MTHFR 677TT genotype

IL-25 regulates Th17 function in autoimmune inflammation

A unique polyphenol-micronutrient blend, A5+, was found to block apoptotic pathways, reduce oxidative stress, and suppress pro-inflammatory cytokines in Parkinsons disease models (387)

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected