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Uptake of CLF To measure CLF polarity, organoids were removed from the Matrigel by washing with PBS and incubated with culture medium supplemented with 10 g/ml CLF (Corning Inc.) and 1 g/ml Hoechst 33342 for 30 minutes at 37 C in a 5% CO 2 incubator

Regularly evaluate patients for signs and symptoms of OIED (e.g., dysphagia, regurgitation, non-cardiac chest pain) and, if necessary, adjust opioid therapy as clinically appropriate (see CLINICAL PHARMACOLOGY)

These metabolites exert their effects not only within the gut but also across various organs due to their capacity to enter systemic circulation

PMID: 23472308