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In contrast, Cacna1c siRNA-transfected cells mostly maintained their tubular, elongated morphology despite the glutamate challenge

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This is the theoretical basis for endogenously supplied gamma-glutamylcysteines ability to raise GSH levels, as being the product of the GCL enzyme, it can be taken up by cells intact, where it feeds directly into glutathione synthase to be converted to glutathione, effectively bypassing the dysfunctional GCL

Subsequently, the cells were carefully rinsed with JC-1 staining buffer to remove excess dye