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10.1093/aje/kwn409 161 SchaeferL.Trujillo-VargasC

While it can lead to various benefits, its important to remember that it is still a form of stress and has a cost (22, 23)

Certain of these previous reports were able to pinpoint the specific cysteine residues which were modified in the GSTs, namely Cys50 in rat and human MGST1 (Shin et al., The analysis of proteins and their modifications benefits from continual advancements in liquid chromatography-tandem mass spectrometry (LC-MS/MS), a broadly used tool for proteomic investigations (Aebersold and Mann, In this work, several analytical approaches (Figure 2) were investigated combining different techniques for sample preparation and LC-MS/MS analysis to identify APAP-related covalent binding to purified human GSTs (overview in Table 1, note: Met1 is considered here as the first amino acid in protein sequences throughout the whole manuscript)

5-RACE was performed using the following primers: AsGGT1-5-RACE-RT (5-[Phos]TCTTCTGAACCG-3), AsGGT1-5-RACE-F1(5-TGCTCTCACCACTCTGTTC-3), AsGGT1-5-RACE-F2 (5-GACTCCATCTCTCATCAGTTC-3), As GGT1-5-RACE-R1 (5-TCACGAACGATGAGCGATG-3), and AsGGT1-5-RACE-R2 (5-CCAGTTTCTGATCAGAAGAAGC-3) for AsGGT1