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DSIP, short for Delta Sleep-Inducing Peptide, is a small naturally occurring neuropeptide that was first identified in the 1970s during research into how the brain regulates sleep

Finally, the purified structure was annealed with 10X excess of the oligonucleotide 253.Primer_for_Dig that was partially complementary to the oligonucleotide 386.Template hairpin 45dC, by heating at 95 C for 1 min and cooling down from 80 C to 10 C at a 0.5 C 10 s -1 rate in annealing buffer (10 mM Tris-HCl pH 7.5, and 1 mM MgCl 2 )

The same studies can be located independently on PubMed using the CAS number (1401708-83-5) or the compound name

Hence, we consider BSO-induced lower food intake as a therapeutic effect on polyphagia but not a toxic effect