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Life Sci 71: 30313045

A., Ismail, L

EVs with TfR deficiency lost their iron-chelating capacity and cardioprotective effect in post-MI hearts

The TEV protease and the -mercaptoethanol were added in the pooled fraction with target protein to TEV protease ratio of 1:10 (w/w) and the final concentration of 10 mM, respectively, to cleave 6HFh8 and dialyzed against 1 PBS with 150 mM NaCl at 4 C overnight