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Du Q, Zhang S, Li A, Mohammad IS, Liu B, Li Y

Essentially, AAV particles of the PHP.eB capsid (serotype), known to efficiently transduce the central nervous system via intravenous injection 42 , expressing Cre recombinase driven by the astrocyte-specific short GFAP promoter (PHP.eB-AAV-gfaABC 1 D-Cre-GFP) were administered intravenously (50 l aliquots of a phosphate-buffered saline (PBS) solution containing 0.001% Pluronic F-68, Sigma-Aldrich and 1 10 11 viral genomes, VG) through the retro-orbital sinus to 2-month-old Cpt1a lox/lox male mice under a brief sevoflurane anaesthesia (Sevorane, at 6% for initiation followed by roughly 3% for maintenance in air with supplements of O 2 and NO 2 0.4 and 0.8 l min 1 , respectively, using a gas distribution column, Hersill H-3, and a vaporizer, InterMed Penlons Sigma Delta)

Resveratrol modulates the gut microbiota and inflammation to protect against diabetic nephropathy in mice

After that, cells from the top of the chamber membrane were gently removed and the invasion cells on the bottom of the membrane were fixed with 4% paraformaldehyde and stained with 0.1% crystal violet (Solarbio)