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These symptoms generally improve by week 10-12 as the body adapts to maintenance dosing

Thus, disruptions caused by Mg deficiency or imbalance in these pathways can adversely affect plant growth, development, and yield (Zhang et al., 2023)

After washing with PBS containing 0.3% Tween 20 (Fisher Scientific, Waltham, MA, United States), the NS3-NS5 interaction was detected by incubating the plates with the anti-NS3 monoclonal antibody (GT2811 clone, Genetex, Irvine, CA, United States) diluted 1:6,000 in PBS + 2% (v/v) FBS (Thermo Fisher Scientific) for 1 h at RT

It has been suggested that glutaminases form oligomeric filament-like structures essential for their activation