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Following fixation, L3 filets were washed in PTX (PBS and 0.02% Triton X-100) and blocked in PBT (PBS, 0.02% Triton X-100 and 1% NGS) on a nutator and incubated with primary antibodies for either 2 h at room temperature or overnight at 4 C with agitation

The most commonly observed irAEs, particularly in treatments combining immune checkpoint inhibitors with chemotherapy, include arthralgia, thyroid dysfunction (both hypothyroidism and hyperthyroidism), transaminitis, dermatologic toxicities, pneumonitis, and colitis

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Khan F, Moinuddin, Mir AR, Islam S, Abidi M, Husain MA, et al