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Cells were resuspended in the same medium, rested at 37 C for 0.51 h, filtered through 100-m strainers (VWR) and dispensed at 1 10 5 neutrophils per well (500 l) in 24-well ultra-low attachment plates

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The conditions of real-time PCR were as follows: (1) initial PCR activation 95 C, 10 min, (2) 3-step cycling for 35 cycles involving (a) denaturation 95 C, 30 s, (b) annealing 58 C, 1 min and (c) extension 72 C, 1 min, followed by a dissociation phase