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After blocking with 5% non-fat milk (BioRad) or bovine serum albumin (BSA) (Sigma-Aldrich), in tris-buffered saline, 0.1% Tween 20 (TBST), membranes were incubated with primary antibodies at 4C overnight

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The patient described in Aretaeus's work had stomach pain and was atrophied, pale, feeble, and incapable of work

426 Moreover, pharmacological compounds, such as Simvastatin, 428 Atorvastatin, 429 Prednisolone, 430 Nicorandil, 431 Treprostinil, 432 Baicalein, 216 exert their effects by suppressing NF-B expression, preventing vascular remodeling and PH