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Herein the formulation and processing parameters in the DoE (Table S3) included the ratio between mannitol and nanoparticle (w/w) (A), the atomization gas flow rate (L/h) (B), and the feed rate of solution during the spray drying process (mL/min) (C), while the responses included redispersibility of the dry powder (Z 1 ), PDI of the reconstituted nanosuspension (Z 2 ), and volumetric particle size of dry powder (m) (Z 3 )

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Assays of PatA and PatD Plasmids pET-patA and pET-patD were transformed into E