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4.5.3.2 Principal factors influencing biomarker application Impact of renal functional status : when glomerular filtration rate declines, multiple metabolic intermediates accumulate systemically, including branched-chain amino acids and aromatic amino acid catabolites, rendering differentiation between authentic pathogenic markers and consequences of diminished renal clearance capacity difficult (50, 51)

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Each concentration was tested in triplicate, with each plate also including untreated controls along with a blank cell-free control

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