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Afterwards, cells were washed twice with PBS, and then incubated for 20 min at room temperature with Rho123 diluted in respiration buffer (130 mM KCl, 5 mM K 2 HPO 4 , 20 mM MOPS, 2.5 mM EGTA, 3 mM succinate, 0.1% BSA, adjusted to pH 7.15 with KOH) to a final concentration of 1 M