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10.3389/fpls.2020.01256 56 LiX.HoltR

The maximum BCF was found to be 4, with a half-life in tissues of 1 day

Single colonies obtained at the highest possible antibiotic concentration were picked and tested for successful deletion of the target gene by colony PCR using suitable flanking primers, followed by double-stranded DNA sequencing of the amplified region (Eurofins Genomics)